Mushroom Grow Kit Instructions: Monotub

At RA Mushrooms, our mission is to educate, innovate, and advocate for the responsible cultivation of psilocybin mushrooms while supporting their research and integration into mental health therapies—not just in Canada, but globally. We are committed to providing cultivators with the highest-quality tools and information to ensure success in their mycology journey.

Thank you for purchasing one of our premium Grow Kits. If you experience any issues before starting, please contact our team immediately so we can address and resolve them promptly.

This Grow Kit introduces a groundbreaking approach to home cultivation, using sterile grainspawn as the foundation for successful mushroom propagation. By utilizing aseptic techniques, users will inoculate the grainspawn with a 10mL syringe of liquid culture mycelium. Each grainspawn bag is designed with a self-healing injection port, allowing for a seamless, contamination-resistant inoculation process.

To ensure success, have the following items ready:

• Nitrile gloves (for sterile handling)
• Dust mask or equivalent (to minimize airborne contaminants)
• 70% Isopropyl alcohol or disinfectant spray (for sterilization)
• Paper towels (for surface cleaning)

Selecting a Clean Work Area:

• Choose a draft-free room, such as a bedroom or kitchen.
• Shut off fans, HVAC systems, and any other sources of airflow.
• Keep all doors closed to reduce the risk of airborne contaminants.

1. Sanitize Your Workspace:
   • Wipe down your work surface, the outside of the grainspawn bag, and the injection port with 70% isopropyl alcohol.
   • Let all surfaces dry completely before proceeding.

1. Attach the Needle to Your Syringe:
   • Ensure a secure connection before removing the syringe cap.

1. Sterilize the Needle:
   • Use a lighter to heat the metal syringe tip until it glows red-hot.
   • Allow the needle to cool for 3-5 seconds.
   • DO NOT WIPE OR TOUCH THE NEEDLE AFTER FLAME STERILIZATION.

1. Inject the Liquid Culture:
   • Insert the cooled syringe needle into the self-healing injection port.
   • Inject 5-10cc of liquid culture into the grainspawn bag.
   • If using only one bag, up to 10cc may be injected, but this is not strictly necessary.

1. Seal & Store the Syringe:
   • If reusing, recap the needle and store it properly.
   • If not reusing, dispose of the needle safely and place the syringe in a recycling container.

• Days 1-7: Mycelium should begin colonization.
• Days 7-10: Shake the bag gently to distribute growth evenly.
• Days 21+: If fully colonized, proceed to the next stage.
• Maintain an incubation temperature of 21-24°C (70-76°F) for optimal results.
• Avoid handling the filter patch to prevent contamination.
• If colonization takes longer than expected, be patient—growth rates vary.

1. Sanitize the Microppose Monotub Grow Kit thoroughly.
2. Place the liner evenly inside the tub.
3. Install the 2” Microppose PLUGS over the side and end ventilation holes.
4. Break up the colonized grainspawn inside the bag and cut open the top.
5. Pour the grainspawn into the tub and mix it with the substrate using sanitized, gloved hands or sterile utensils.
6. Smooth down the surface to create an even layer, similar to spreading cake batter.
7. Secure the lid and place the kit in an undisturbed environment, away from direct sunlight.

• 48 Hours Post-Setup: Mycelium should start colonizing the substrate.
• Days 10-12: Remove the Microppose PLUGS and replace them with Microppose Air Exchange Filters to initiate pinning.
• Maintain High Humidity (~90%) inside the tub.
• Evaporation is key for triggering mushroom pinning—mist lightly as needed.

Daily Monitoring:

• Check for moisture levels and adjust misting accordingly.
• Avoid excessive misting if condensation and moisture beads are already present.

• Harvest mushrooms before caps fully open to prevent spores from dropping.
• Use alcohol-sanitized scissors or a scalpel to cut mushrooms at the base.
• Inspect the tub for contamination before opening it.

• After harvesting, replenish moisture by pouring 2 cups of cold water onto the substrate or heavily misting the surface.
• Let it sit for 4-6 hours, then drain any excess water.

Your Microppose Monotub Grow Kit is reusable, allowing for multiple cultivation cycles with any mushroom variety of your choice.

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