Mushroom Grow Kit Instructions: BAGTEK

At RA Mushrooms, we are dedicated to educating, innovating, and advocating for responsible psilocybin cultivation while supporting research into its mental health applications—not just in Canada, but globally. Our goal is to provide cultivators with high-quality tools and knowledge for success.

Thank you for purchasing one of our premium Grow Kits! If you encounter any issues before starting, please contact our team immediately so we can assist you.

This Grow Kit utilizes a groundbreaking process by starting with sterile grainspawn. This method allows cultivators to apply aseptic techniques when inoculating the grainspawn using a 10mL syringe of liquid culture mycelium. Each grainspawn bag features a self-healing injection port, ensuring a seamless and contamination-resistant process.

Prepare the following items before starting your grow kit:

✔ Nitrile gloves (for sterile handling)
✔ Dust mask or equivalent (to reduce airborne contaminants)
✔ 70% Isopropyl alcohol or disinfectant spray (for sterilization)
✔ Paper towels (for surface cleaning)

• Choose a draft-free room (e.g., bedroom or kitchen).
• Turn off fans, HVAC systems, and any other sources of airflow.
• Keep all doors closed to minimize airborne contaminants.

1. Sanitize Your Workspace:
   • Wipe down your work surface, the outside of the grainspawn bag, and the injection port with 70% isopropyl alcohol.
   • Allow all surfaces to dry completely before proceeding.

1. Attach the Needle to Your Syringe:
   • Ensure a secure connection before removing the syringe cap.

1. Sterilize the Needle:
   • Use a lighter to heat the metal syringe tip until it glows red-hot.
   • Allow the needle to cool for 3-5 seconds.
   • DO NOT WIPE OR TOUCH THE NEEDLE AFTER FLAME STERILIZATION.

1. Inject the Liquid Culture:
   • Insert the cooled syringe needle into the self-healing injection port.
   • Inject 5-10cc of liquid culture, moving the needle slightly while injecting.
   • If using only one bag, up to 10cc may be injected, though this is not strictly necessary.

1. Seal & Store the Syringe:
   • If reusing, recap the needle and store it properly.
   • If not reusing, dispose of the needle safely and place the syringe in a recycling container.

• Days 1-4: Mycelium should begin colonization.
• Days 5-10: Allow further growth before gently shaking the bag to promote even colonization.
• Days 21+: If fully colonized, proceed to the next stage.
• Maintain an incubation temperature of 21-24°C (70-76°F) for optimal results.
• Avoid handling the filter patch to prevent contamination.
• If colonization takes longer than expected, be patient—growth rates vary.

Once colonization is complete, it's time to set up your Bagtek Grow Kit!

1. Sanitize Your Hands & Work Area:
   • Wear nitrile gloves and wipe them down with isopropyl alcohol.
   • Sanitize the spawn and substrate containers before opening.

1. Combine Spawn & Substrate:
   • Open both containers and pour their contents into the designated fruiting bag.

1. Mix Thoroughly & Secure the Bag:
   • Fold the top of the bag twice and shake well to evenly mix the spawn and substrate.
   • Break apart any mycelium clumps to ensure even dispersion.
   • Lightly tamp the bag on a flat surface to settle the substrate.
   • Fold the opening twice and secure it with paperclips to prevent accidental openings and retain moisture.

Optional Step:

• Use the provided elastic band to wrap around the Bagtek Grow Kit ½ inch below the surface.
• This can enhance yield concentration and simplify harvesting.

Once your kit is set up, clean your workspace and place the Bagtek Grow Kit in a stable environment with minimal airflow, away from direct sunlight.

• Within 48 hours, mycelium should begin spreading.
• Days 10-12: The substrate will fully consolidate.
• Briefly open the bag or lid for fresh air exchange.
• If the surface appears dry, lightly mist inside the bag to restore lost humidity.

Daily Monitoring:

• Look for tiny water beads along the surface—this indicates proper humidity.
• Avoid excessive misting; the goal is to maintain evaporation cycles for optimal mushroom formation.
• After 3-7 days, small hyphal knots will appear, eventually developing into pins—the beginning stage of your mushrooms.

• Harvest mushrooms before the caps fully open to prevent excessive spore drop.
• Inspect the substrate for any signs of contamination before fully opening the bag.
• To harvest: 
  • Either pull the bag down or cut it open for easy access.
  • Use alcohol-sanitized scissors or a scalpel to cut mushrooms at the base.
  • Alternatively, twist and pull them off the substrate, though some substrate may come off with them.

• After harvesting, replenish moisture by pouring ½ cup of cold water over the substrate or misting heavily.
• Let it sit for 4-6 hours, then drain any excess water.

Your Bagtek Grow Kit can be flushed multiple times, as long as there is no contamination present.

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Thank you for supporting RA Mushrooms—your journey into mycology starts here! 🍄